Vitamin D Receptor Gene Polymorphisms and the Risk of Chronic Hepatitis C Related Hepatocellular Carcinoma in Egyptian Population

Background: Small percentage of hepatitis C (HCV) patients develop hepatocellular carcinoma (HCC) during their lifetime, suggesting that genetic factors might modulate HCC development. Numerous variations on the vitamin D receptor gene (VDR) have been recognized in human cancers. The majority them cause VDR to be unable bind 1, 25-OH-D. aim present work was investigate relation FokI (rs2228570), BsmI (rs3782905) and ApaI (rs7975232) polymorphisms risk development chronic HCV Egyptian patients. Methods: A total 311 were enrolled for this study. They divided into 3 groups: 103 with liver Cirrhosis, 107 101 normal healthy subjects as control group. Human genomic DNA Extraction carried out using QIAamp® Blood Mini Kit (QIAGEN) Genotyping single nucleotide polymorphism (SNP) real-time PCR TaqMan allelic discrimination assay allele-specific designed fluorescent MGB probes. Results: Patients had a higher frequency CC genotype (P=0.035) CI (0.031-0.038). ratio compared those cirrhosis (x 2 =5.4 P = 0.03) or controls =6.8 0.01). Univariate analysis revealed age, lower platelet count (<150×10 /μL), AFP (>100 ng/ml), significantly associated HCC. Stepwise logistic regression showed all independent predictors. Conclusion: mutation is an factor Cirrhotic